Composite

Part:BBa_K3385061:Design

Designed by: Daniel Bavnh�j   Group: iGEM20_DTU-Denmark   (2020-10-16)


T1G3R_SxlnD_glaA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 5777
    Illegal EcoRI site found at 7205
    Illegal XbaI site found at 1623
    Illegal XbaI site found at 5808
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
    Illegal PstI site found at 4039
    Illegal PstI site found at 6615
    Illegal PstI site found at 8349
    Illegal PstI site found at 8950
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 5777
    Illegal EcoRI site found at 7205
    Illegal NheI site found at 4916
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
    Illegal PstI site found at 4039
    Illegal PstI site found at 6615
    Illegal PstI site found at 8349
    Illegal PstI site found at 8950
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 5777
    Illegal EcoRI site found at 7205
    Illegal BglII site found at 3894
    Illegal BglII site found at 5416
    Illegal BglII site found at 8447
    Illegal BamHI site found at 1729
    Illegal BamHI site found at 2157
    Illegal BamHI site found at 4874
    Illegal BamHI site found at 5676
    Illegal BamHI site found at 5802
    Illegal BamHI site found at 6353
    Illegal XhoI site found at 548
    Illegal XhoI site found at 735
    Illegal XhoI site found at 963
    Illegal XhoI site found at 6213
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 5777
    Illegal EcoRI site found at 7205
    Illegal XbaI site found at 1623
    Illegal XbaI site found at 5808
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
    Illegal PstI site found at 4039
    Illegal PstI site found at 6615
    Illegal PstI site found at 8349
    Illegal PstI site found at 8950
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 5777
    Illegal EcoRI site found at 7205
    Illegal XbaI site found at 1623
    Illegal XbaI site found at 5808
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
    Illegal PstI site found at 4039
    Illegal PstI site found at 6615
    Illegal PstI site found at 8349
    Illegal PstI site found at 8950
    Illegal NgoMIV site found at 2566
    Illegal NgoMIV site found at 5134
    Illegal NgoMIV site found at 8337
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3422
    Illegal BsaI site found at 3560
    Illegal BsaI site found at 4249
    Illegal BsaI.rc site found at 4742
    Illegal SapI site found at 3140
    Illegal SapI site found at 8838
    Illegal SapI site found at 9147


Design Notes

Full construct made using non-overlapping primers, which can pose difficulties on PCR amplification and subsequently USER assembly.

Source

Sequences were obtained using aspgd.org[1] and SingalP-5.0[2].

References

[1] Cerqueira GC, Arnaud MB, Inglis DO, Skrzypek MS, Binkley G, Simison M, Miyasato SR, Binkley J, Orvis J, Shah P, Wymore F, Sherlock G, Wortman JR (2014). The Aspergillus Genome Database: multispecies curation and incorporation of RNA-Seq data to improve structural gene annotations. Nucleic Acids Res 42 (1); D705-10.

[2] SignalP 5.0 improves signal peptide predictions using deep neural networks. José Juan Almagro Armenteros, Konstantinos D. Tsirigos, Casper Kaae Sønderby, Thomas Nordahl Petersen, Ole Winther, Søren Brunak, Gunnar von Heijne and Henrik Nielsen. Nature Biotechnology, 37, 420-423, doi:10.1038/s41587-019-0036-z (2019)